Summary Round #3

This round I am reconstituting each injection fresh (instead of making one big batch and refrigerating). I also restarted my workouts. Take 2g of NMN (sporadically), 1 tablespoon of C60OO (sporadically), and have reduced the amount of gluten in my diet.

Jan.17th- injected 30mg

Jan.20th- injected 30mg

Jan.25th- injected 30mg, tied my best treadmill record (from the year 2000!), (6 incline, 6 mph, 6 mins)

Jan.28th- injected 20mg

Jan.31st- injected 20mg

Feb.2nd- Damn, I’m feeling great! and hit new all time treadmill record, (6 incline, 6 mph, 6.1 mins)

Again, I’ve noticed a chg in my sleep patterns -where I seem to need less sleep. Have also noticed muscle cramps.

Latest Blood work:

 

 

 

 

 

 

 

 

 

 

 

New DNA Bio-age:

 

 

 

 

 

 

 

 

 

 

 

 

Blood factor analysis:

35 Comments


  1. Looking forward to hearing from you Darren.
    Ciao Marcello

    Reply

  2. Hi,

    Has you hair remained thicker and darkers?

    Thanks!

    Reply

    1. hi, actually it seems to thinning now. I haven’t taken any foxo4-dri since Oct.
      Hopefully, this next round will show an improvement again (I don’t care much about my appearance–just would like more evidence that FOXO4-DRI is working). Will start Round 3 tomorrow.

      Reply

      1. Thanks, just asking because it is more of an easily observable change.

        Good luck with round 3!

        Reply

  3. Did you notice a reduction in gray hairs during your first and second rounds?

    Since you work in the “industry” do you know what kind of peptide synthesizer is used for production?

    Since a side effect of FOX04-DRI is a reduced ability to heal wounds did you notice an increased level of soreness from any of your exercising? If I were to self experiment would I need to take some time off from weight training?

    Reply

  4. If you also take NMN 2 g per day, how will you say it is due to Foxo4dri or NMN?

    Reply

    1. Yes. Or if the latest tests that come out showing improvements (I assume they will!) are because I’m now working out, or using C60 and Olive oil, or because I’m cutting out wheat from my diet. Or was it a combination of senolytic therapy and any of these. Frankly, there are too many variables in a human to do a controlled study. As an aside, I wonder if actually drawing blood more often for tests(and donations) is helpful to remove some of the systemic pro-aging factors accumulating.
      As a another aside, because the 3 gene markers selected for DNA analysis were selected because they give the best correlation to chronological age– is it going to be admissible in giving an indication of biological age?

      Reply

  5. Any progress with your round 3? Is your hair thickening again? How about general well being?

    Reply

    1. Yes! very exciting, can’t wait until tomorrow.

      Reply

  6. Hi, The study did show good outcomes with mouse kidney. I saw no change with my kidney related blood markers except for BUN which did show an improvement only after the last round, and who knows -maybe I was just more hydrated. I certainly can’t recommend anyone taking this very untested substance.

    Reply

  7. Have you received DNA bio age result?

    Reply

    1. Still waiting. Apparently the first time they tried came back inadmissable

      Reply

  8. Hello Darren. Questions –

    1. When you inject 30 mg, how much saline due to reconstitute it in ?

    2. Where do you usually inject it in ?

    Thanks !

    Richard

    Reply

    1. hi,
      1. about 1ml, it dissolves very readily.
      2. subcutaneous in belly flank fat.

      Reply

  9. Thanks.

    I’ve been looking for info as to whether elevated levels of testosterone in TRT men would inhibit any effects of the injected peptide.

    Have you come across any discussion / info in this regard ?

    Reply

    1. Well, to get the full benefits of a senolytic it is important not to inhibit SASP prior to administration… and anti-inflammatories repress NF-kB — which inhibit SASP.

      Testosterone effects a lot of stuff down stream. it increases both mTOR phosphorylation (can be blocked by rapamycin) and both ERK1/2 and Akt phosphorylation.
      And it promotes p38, and downregulates NF-kB ( https://www.ncbi.nlm.nih.gov/pubmed/19169266 )
      So best to not use testosterone prior to a senolytic.

      Interestingly, Metformin may be an excellent drug to take before a senolytic because it ‘lowers the bar’ for cells with DNA damage to become senescent (https://www.tandfonline.com/doi/pdf/10.4161/cc.11.6.19665).

      Reply

      1. Thank you so much for the info Darren !

        Richard

        Reply

      2. Thanks again Darren.

        I was thinking that after Fox04 injection, testosterone replacement therapy can be restarted 2 weeks later.

        Any thoughts ?

        Richard

        Reply

        1. T has so many downstream affects – dunno what’s best to do with this combo. I restarted my Testosterone in the middle of Cycle 3…

          Reply

  10. Your Bio Age results indicate 52.5 years. So it went back up after the latest round?

    Reply

    1. Yes, and it became 4 times more accurate. Because they pick the genes that correlate with chrono age, this test is actually not very useful at determining if my bio age has improved. For example my blood work has improved a lot– heck, I used to have Metabolic disease but that has disappeared.

      Reply

  11. Thanks for doing the hard yards.
    Amazing work.
    Please keep us informed

    Reply

  12. What is the latest today? Are you alive? Are you well? Did you take more Fox peptide?

    Reply

    1. Alive and well. Thx!
      In fact, today I’m doing another short interview for a Belgian TV station. They will tape me giving a blood donation and talk to me about my Optimal Longevity Protocol that I’ve been working on. I will take a shot of FOX04-dri -but the TFA salt free version—FYI: TFA salt is somewhat toxic and could have been behind the mosquito-bite like reaction myself and others experience. If you do not ask for TFA salt free then you will get with TFA.

      Reply

  13. I want to be a tester of your products if somebody can help me. I am 21 years old want to live to 200+….i think foxo4 dri is the one.

    My email: [email protected]

    Reply

  14. I placed an order with Breakthrough Labs, one of your listed sources. I have not received my order. I have attempted to reach them multiple times via their telephone number and chat function and reach only an automated response system. No one will return my call or email request.

    Do you have any knowledge of this ?

    Reply

    1. hi, dunno, but one fellow that was involved with Breakthrough Labs died a few mths ago…
      Thanks for the info. and pls let me know in a couple weeks if you still have no product or correspondence, and I’ll add a warning about them…

      Reply

  15. Hi Darren,

    Just did the Young.AI analysis and came up with a biological age of 33 when my chronological age is 64. That is encouraging since my epi age is 65 and telomere age is 66 for median length but 54 for average length. I agree that epi measures might not be useful since many epi changes are positive e.g., for cancer expression.

    I am including my Young.AI results:

    Test CountBlood TestValueReference Range
    1Albumin4.83.5 – 5.5 U/L
    2Alkaline phosphatase6339 – 117 U/L
    3ALT19< 42 U/l
    4AST35< 40 U/l
    5Basophils1< 1.0 %
    6Bilirubin total0.40.0 – 1.2 mg/dL
    7BUN – urea226 – 24 mg/dL
    8Calcium9.68.7 – 10.2 mg/dL
    9Chloride10197 – 108 mmol/L
    10Cholesterol189100 – 199 mg/dL
    11Creatinine10.57 – 1.00 mg/dL
    12Eosinophils0.1< 5.0 %
    13Globulins2.21,4 – 4,9 g/dl
    14Glucose9065 – 99 mg/dL
    15HDL Cholesterol103> 39 mg/dL
    16Hematocrit49.437 – 50 %
    17Hemoglobin16.211.1 – 15.9 g/dL
    18Iron5635 – 155 ug/dL
    19Lactate dehydrogenase18919 – 226 IU/L
    20LDL Cholesterol720 – 99 mg/dL
    21Lymphocytes2120 – 40 %
    22MCH31.226.6 – 33.0 pg
    23MCHC32.831.5 – 35.7 g/dL
    24MCV9579 – 97 fL
    25Monocytes103 – 9 %
    26Neutrophils6745 – 70 %
    27Platelet Count210150-379 103 /uL
    28Potassium4.53.5 – 5.2 mmol/L
    29Protein total76.0 – 8.5 g/dL
    30RBC – red blood cells (Erythrocytes)5.23.77 – 5.28 106 /uL
    31Sodium142134 – 144 mmol/L
    32Triglycerides720 – 149 mg/dL
    33WBC – white blood cells (Leukocytes)7.23.4-10.8 103 /uL

    Reply

    1. hey, thanks for posting real data

      Reply

      1. I wonder how accurate Young.AI really is. My dashboard there says that my triglycerides are critically low but they are at 72, not a great number but normal. That could just be their conversion factor (I think they use index numbers) for their graphics or could be an underlying issue.

        BTW I just started on a new round, the second in a year, of FOXO4-DRI. Every other day for 15~ days, I think. I have been tired as crap. I hope my next round of blood tests will show a drop in homocysteine and other markers of inflammation. Since nothing else will change in my regimen I will also be measuring telomere length for the 4th time and the 3rd time with LifeLength and I hope to see a statistically significant increase in telomere length which could indicate that my body’s regenerative capacity has increased and thereby amplifying the effectiveness of FOXO4-DRI as a senolytic. I have been taking 32mg of TA-65 daily for 9 months.

        Another measure of the effectiveness of FOXO4 could be exercise capacity. Those measures are all being done as well. Since FOXO4 is the only variable here we’ll see if we could associate positive changes out of the ordinary with FOXO4. I’ll report back in December.

        Cheers!

        Reply

  16. Hi Darren. Do you have a link to the Belgian TV program or else the name of that program? Thanks and good luck!

    Reply

    1. hi, I found out it’s air date ETA is Jan. ’19

      Reply

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